Resistance of alkylated deoxyribonucleic acid to nuclease attack
نویسندگان
چکیده
منابع مشابه
Mechanism of action of micrococcal nuclease on deoxyribonucleic acid.
Two types of methods have been used for the elucidation of specificity (or preference) of nucleases when complex polynucleotides are used as substrates. One method depends on the identification of products in the digest. I f a particular combination (or combinations) is missing, it means that this combination is preferentially attacked. Thus, in the case of pancreatic ribonuclease, sequences Py...
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Deoxyribonucleic acid (DNA)-DNA hybridization on nitrocellulose filters can be used to assay for replication origin DNA from Escherichia coli if the DNA attached to the filters is enriched for the replication origin sequences. Such DNA can be readily isolated from very rapidly growing cells. When low amounts of this DNA were attached to filters, radioactively labeled DNA from the replication or...
متن کاملIsolation of covalently closed circular deoxyribonucleic acid from bacteria which produce exocellular nuclease.
Reproducible yields of covalently closed circular (plasmid) deoxyribonucleic acid were obtained from mutants defective for extracellular nuclease but not from the corresponding wild-type strain of Serratia marcescens
متن کاملDeoxyribonucleic acid-deoxyribonucleic acid hybridization of R factors.
A new method of estimating deoxyribonucleic acid homology is presented and used to measure the homology between bacterial plasmids of different compatibility groups. Deoxyribonucleic acid of the P-group plasmid RP1, originally isolated in Pseudomonas aeruginosa, has considerable (50%) homology with an N-group plasmid of the Enterobacteriaceae.
متن کاملEnzymatic Synthesis of Deoxyribonucleic Acid. Deoxyribonucleic Acid By
MISSOURI The enzymatic synthesis of DNA1 in sufficient excess over the DNA used to prime the reaction21 a has enabled us to examine the chemical composition of the synthetic product. As has already been demonstrated, this DNA consists of deoxy-nucleotides Linked by typical 3'-5' phosphodiester bonds, and all four of the deoxy
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1972
ISSN: 0306-3283
DOI: 10.1042/bj1280138p